Browsing by Author "Méndez Albores, Alia"
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Artículo A review on SERS-based detection of human virus infections: influenza and coronavirus(2021-02-28) Méndez Albores, Alia; González Fuentes, Miguel A.; López-Castaños, Karen A.; Carabarin Lima, Alejandro; López Castaños, Mónica; Méndez, Erika; Saviñon Flores, Fernanda; Méndez Albores, Alia; 0000-0002-9047-8766; González Fuentes, Miguel A.;0000-0003-0630-151X; Carabarin Lima, Alejandro; 0000-0002-0162-4929; Méndez, Erika; 0000-0001-6859-8959"The diagnosis of respiratory viruses of zoonotic origin (RVsZO) such as influenza and coronaviruses in humans is crucial, because their spread and pandemic threat are the highest. Surface–enhanced Raman spectroscopy (SERS) is an analytical technique with promising impact for the point–of–care diagnosis of viruses. It has been applied to a variety of influenza A virus subtypes, such as the H1N1 and the novel coronavirus SARS−CoV−2. In this work, a review of the strategies used for the detection of RVsZO by SERS is presented. In addition, relevant information about the SERS technique, anthropozoonosis, and RVsZO is provided for a better understanding of the theme. The direct identification is based on trapping the viruses within the interstices of plasmonic nanoparticles and recording the SERS signal from gene fragments or membrane proteins. Quantitative mono- and multiplexed assays have been achieved following an indirect format through a SERS-based sandwich immunoassay. Based on this review, the development of multiplex assays that incorporate the detection of RVsZO together with their specific biomarkers and/or secondary disease biomarkers resulting from the infection progress would be desirable. These configurations could be used as a double confirmation or to evaluate the health condition of the patient".Artículo Electrochemical biosensor for sensitive quantification of glyphosate in maize kernels(2019-02-12) Méndez Albores, Alia; Cahuantzi Muñoz, Selene L.; González Fuentes, Miguel A.; Ortiz Frade, Luis A.; Torres, Eduardo; Ţălu, Ştefan; Trejo, G.; Méndez Albores, Alia; 0000-0002-9047-8766; González Fuentes, Miguel A.; 0000-0003-0630-151X; Ştefan Ţălu, Ştefan Ţălu; 0000-0003-1311-7657"A graphite-epoxy electrode (GE) modified with multiwalled carbon nanotubes (MWCNTs) and horseradish peroxidase (GE/MWCNTs-HRP) was used to build a glyphosate biosensor whose performance in aqueous solutions depends on the enzyme activity. For the biosensor preparation, MWCNTs were deposited onto the GE surface by electrophoresis using an oxidative treatment (H2SO4/HNO3) in presence of cetyl tributylammonium bromide (CTAB) as a cationic surfactant. The surfactant was further removed from the MWCNTs surface by dipping the electrode in an EtOH/HCl solution. The physical immobilization of HRP and therefore the glyphosate sensing capabilities was tested at pH 4 where the herbicide exhibits one only species. Circular dichroism studies suggested that the secondary structure of HRP changes as a result of its interaction with glyphosate and that this change is intensified by the combination of glyphosate and H2O2, which may explain the decrease of the enzyme catalytic activity with the increase of glyphosate concentration. The glyphosate quantification in doped-maize kernels was highly reproducible and exhibits detection and quantification limits of 1.32 pM and 1.63 pM respectively. The biosensor is also characterized by a high recovery (100 %) and precision (coefficient of variation <1 %) and can be employed in presence of interfering substances such as chlorpyrifos (an organophosphate pesticide) and starch".Artículo Enfermedad de chagas: crónica de un mal olvidado(2022-06-18) Ortega Caballero, Miguel; Trejo, Gabriel; Carabarín Lima, Alejandro; Méndez Albores, Alia; Ortega Caballero, Miguel; 0000-0002-3744-1462; Trejo, Gabriel; 0000-0002-3744-1462; Carabarín Lima, Alejandro; 0000-0002-0162-4929; Méndez Albores, Alia; 0000-0002-9047-8766"La Tripanosomiasis americana o también conocida como enfermedad de Chagas, es una enfermedad ocasionada por el parásito protozoario Trypanosoma cruzi (T. cruzi). El nombre particular de esta enfermedad se debe al apellido del médico-investigador brasileño que la descubrió en el año de 1909, Carlos Ribeiro Justiniano das Chagas. Este mal es una enfermedad zoonótica vectorial, esto quiere decir que el parásito T. cruzi se tramite al hombre a través de insectos hematófagos, mejor conocidas como chinches besuconas, las cuales pertenecen a la familia Reduviidae y los géneros Rhodnius, Triatoma y Pastrongylus. En México, se presentan un total de 29 especies distintas de estos géneros, de las cuales 21 se encuentran infectadas con T. cruzi, por lo que el riesgo de trasmisión es alto en áreas endémicas".Artículo Indirect quantification of glyphosate by SERS Using an incubation process with hemin as the reporter molecule: a contribution to signal amplification mechanism(2020-12-18) López Castaños, Karen Alejandra; Ortiz Frade, Luis A.; Méndez, Erika; Quiroga González, Enrique; González Fuentes, Miguel Angel; Méndez Albores, Alia; López Castaños, Karen Alejandra; 0000-0002-7585-3505; Méndez, Erika; 0000-0001-6859-8959; Quiroga González, Enrique; 0000-0003-1650-0862; González Fuentes, Miguel A.; 0000-0003-0630-151X; Méndez Albores, Alia; 0000-0002-9047-8766"The indirect determination of the most used herbicide worldwide, glyphosate, was achieved by the SERS technique using hemin chloride as the reporter molecule. An incubation process between hemin and glyphosate solutions was required to obtain a reproducible Raman signal on SERS substrates consisting of silicon decorated with Ag nanoparticles (Si-AgNPs). At 780 nm of excitation wavelength, SERS spectra from hemin solutions do not show extra bands in the presence of glyphosate. However, the hemin bands increase in intensity as a function of glyphosate concentration. This allows the quantification of the herbicide using as marker band the signal associated with the ring breathing mode of pyridine at 745 cm−1. The linear range was from 1 × 10−10 to 1 × 10−5 M and the limit of detection (LOD) was 9.59 × 10−12 M. This methodology was successfully applied to the quantification of the herbicide in honey. From Raman experiments with and without silver nanoparticles, it was possible to state that the hemin is the species responsible for the absorption in the absence or the presence of the herbicide via vinyl groups. Likewise, when the glyphosate concentration increases, a subtle increase occurs in the planar orientation of the vinyl group at position 2 in the porphyrin ring of hemin over the silver surface, favoring the reduction of the molecule. The total Raman signal of the hemin-glyphosate incubated solutions includes a maximized electromagnetic contribution by the use of the appropriate laser excitation, and chemical contributions related to charge transfer between silver and hemin, and from resonance properties of Raman scattering of hemin. Incubation of the reporter molecule with the analyte before the conjugation with the SERS substrate has not been explored before and could be extrapolated to other reporter-analyte systems that depend on a binding equilibrium process".Artículo Recubrimientos metálicos antimicrobianos(2022-05-28) Trejo, Gabriel; Rios Álvarez, Claudia; Méndez Albores, Alia; Trejo, Gabriel; 0000-0001-8202-2366; Rios Álvarez, Claudia; 0000-0002-6667-8290; Méndez Albores, Alia; 0000-0002-9047-8766"Datos epidemiológicos reportados por la Organización Mundial de la Salud (OMS) indican que las bacterias y virus (microbios) son las principales causantes de la transmisión de enfermedades infecciosas de una persona a otra. Actualmente, las enfermedades causadas por la transmisión de patógenos virales y bacterianos son el principal foco de atención del sector salud en el mundo. La OMS reporta que cerca de 20 mil personas mueren diariamente por este tipo de enfermedades, lo que constituye un serio problema de salud pública a nivel mundial, que con el surgimiento de la pandemia SARS-COV-2 se ha agravado considerablemente, teniendo importantes consecuencias económicas y sociales. La OMS estima que si no se toman acciones preventivas contra los microorganismos patógenos, la humanidad estará expuesta a sufrir infecciones intratables a causa del aumento de la resistencia de bacterias y virus a los antibióticos".