Browsing by Author "Morales Ledesma, Leticia"
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Artículo Polycystic ovary syndrome induced by exposure to testosterone propionate and effects of sympathectomy on the persistence of the syndrome(2017-07-10) Morales Ledesma, Leticia; Díaz Ramos, Juan Antonio; Trujillo Hernández, Angélica; Morales Ledesma, Leticia; 0000-0002-5276-8260; Trujillo Hernández, Angélica; 0000-0002-9931-9294"Polycystic ovary syndrome is a complex disorder affecting 6 to 10% of women of reproductive age. In recent years, the cause of the syndrome has been associated with increased androgen serum levels, as well as sympathetic nervous system hyperactivity. The present study analyzed the effects at birth of a single testosterone propionate dose in rats, as well as the role of the superior ovarian nerve in polycystic ovary syndrome persistence. Newborn female rats of the CIIZV strain were injected with a single dose of 100 μg testosterone propionate, or corn oil as a vehicle. At 24 days of age, rats were subjected to superior ovarian nerve unilateral or bilateral section. A group of animals was sacrificed on their first vaginal estrus, and a second group was sacrificed at 90 days of age, when they presented an estrus preceded by a proestrus".Artículo Sex-specific hypothalamic expression of kisspeptin, gonadotropin releasing hormone, and kisspeptin receptor in progressive demyelination model(2022-09) Muñoz de la Torre, Luz Patricia; Trujillo Hernández, Angélica; Eguibar, Jose R; Cortés, Carmen; Morales Ledesma, Leticia; Muñoz de la Torre, Luz Patricia; 0000-0003-2176-0239; Trujillo Hernández, Angélica; 0000-0002-9931-9294; R Eguibar, Jose R; 0000-0001-6594-1141"Demyelinating diseases, such as multiple sclerosis, decrease the quality of life of patients and can affect reproduction. Assisted reproductive therapies are available, which although effective, aggravate motor symptoms. For this reason, it is important to determine how the control of the hypothalamus-pituitary-gonadal axis is affected in order to develop better strategies for these patients. One way to determine this is using animal models such as the taiep rat, which shows progressive demyelination of the central nervous system, and was used in the present study to characterize the expression of gonadotrophin-releasing hormone (GnRH), Kisspeptin, and kisspeptin receptor (Kiss1R) and luteinizing hormone (LH) secretion. The expression of kisspeptin, GnRH, and Kiss1R was determined at the hypothalamic level by immunofluorescence and serum LH levels were determined by ELISA. The expression of kisspeptin at the hypothalamic level showed sexual dimorphism, where there was an increase in males and a decrease in females during oestrus. There was no change in the expression of GnRH or kisspeptin receptor, regardless of sex. However, a decrease in serum LH concentration was observed in both sexes. The taiep rat showed changes in the expression of kisspeptin at the hypothalamic level".Artículo The neural signals of the superior ovarian nerve modulate in an asymmetric way the ovarian steroidogenic response to the vasoactive intestinal peptide(2018-08-20) Rosas, Gabriela; Linares, Rosa; Ramírez, Deyra A.; Vieyra Valdez, Elizabeth; Trujillo Hernández, Angélica; Domínguez, Roberto; Morales Ledesma, Leticia; Linares, Rosa; 0000-0002-5575-4120; Vieyra Valdez, Elizabeth; 0000-0001-9413-4808; Trujillo Hernández, Angélica; 0000-0002-9931-9294; Morales Ledesma, Leticia; 0000-0002-5276-8260"The superior ovarian nerve (SON) provides neuropeptide-Y, norepinephrine and vasoactive intestinal peptide (VIP) to the ovaries. Ovarian steroidogenesis is modulated by the SON. In the cyclic rat, the acute steroidogenic response to ovarian microinjection of VIP is asymmetric and varies during the estrous cycle. In the present study, we analyze whether the differential effects of VIP in each ovary are modulated by the neural signals arriving through the SON. Cyclic female rats were submitted on diestrus-1, diestrus-2, proestrus, or estrus to a unilateral section of the SON, and immediately afterward, the denervated ovary was either microinjected or not with VIP. Animals were sacrificed 1 h after treatment. The injection of VIP into the left denervated ovary performed on diestrus-1 decreased progesterone levels in comparison with the left SON sectioning group; similar effects were observed on proestrus when VIP was injected into either of the denervated ovaries".Artículo Unilateral section of the superior ovarian nerve induces first ovulation in the Zucker fatty (fa/fa) rat(2021-01-01) Puga y Colmenares, María Concepción; Trujillo Hernández, Angélica; Morales Ledesma, Leticia; Trujillo Hernández, Angélica; 0000-0002-9931-9294; Morales Ledesma, Leticia; 0000-0002-5276-8260"Hyperactivity in the sympathetic nervous system has been shown to be related to the development of ovarian pathologies. In addition, obesity has been found to be associated with multiple reproductive anomalies and is considered a chronic stress condition of low intensity with changes in the peripheral sympathetic activity. Therefore, in the present study, we aimed to evaluate if the information reaching the ovaries through the superior ovarian nerve (SON) modifies the ovarian function of Zucker fatty rats. We performed a unilateral section of the SON at 32 days of age and autopsies were carried out on the day of the first vaginal estrus. The results showed that fatty animals do not ovulate on the day of the first vaginal estrus and exhibit an increase in catecholaminergic fibers and the presence of precystic structures in the ovaries, without changes in the onset of puberty or in the secretion of ovarian and hypophyseal hormones. We also found that the section of the right SON resulted in ovulation on the day of the first vaginal estrus, which was accompanied by a decrease in ovarian noradrenaline content. The section of the left SON caused a delay in puberty without changes in the rest of the parameters".