H-ABC tubulinopathy revealed by label-free second harmonic generation microscopy

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dc.audiencegeneralPublices_MX
dc.contributor.authorCortes Sánchez, Ma. del Carmen
dc.contributor.authorAlata Tejedo, Milvia Iris
dc.contributor.authorPiazza, Valeria
dc.contributor.authorJaramillo Restrepo, Carla
dc.contributor.authorEguibar Cuenca, José Ramón
dc.contributor.authorHernández González, Víctor Hugo
dc.creatorCortes Sánchez, Ma. del Carmen; 0000-0002-9163-2665
dc.creatorAlata Tejedo, Milvia Iris; 0000-0003-0598-4136
dc.creatorPiazza, Valeria; 0000-0001-7160-5486
dc.date.accessioned2023-03-21T16:26:43Z
dc.date.available2023-03-21T16:26:43Z
dc.date.issued2022-08-22
dc.description.abstract"Hypomyelination with atrophy of the basal ganglia and cerebellum is a recently described tubulinopathy caused by a mutation in the tubulin beta 4a isoform, expressed in oligodendrocytes. The taiep rat is the only spontaneous tubulin beta 4a mutant available for the study of this pathology. We aimed to identify the effects of the tubulin mutation on freshly collected, unstained samples of the central white matter of taiep rats using second harmonic generation microscopy. Cytoskeletal differences between the central white matter of taiep rats and control animals were found. Nonlinear emissions from the processes and somata of oligodendrocytes in tubulin beta 4a mutant rats were consistently detected, in the shape of elongated structures and cell-like bodies, which were never detected in the controls. This signal represents the second harmonic trademark of the disease. The tissue was also fluorescently labeled and analyzed to corroborate the origin of the nonlinear signal. Besides enabling the description of structural and molecular aspects of H-ABC, our data open the door to the diagnostic use of nonlinear optics in the study of neurodegenerative diseases, with the additional advantage of a label-free approach that preserves tissue morphology and vitality".es_MX
dc.identifierhttps://doi.org/10.1038/s41598-022-18370-x
dc.identifier.urihttps://hdl.handle.net/20.500.12371/17849
dc.language.isospaes_MX
dc.rights.accesopenAccesses_MX
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0es_MX
dc.titleH-ABC tubulinopathy revealed by label-free second harmonic generation microscopyes_MX
dc.typeArtículoes_MX
dc.type.conacytarticlees_MX
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